Mechanism of Binding of Reduced Nicotinamide Adenine Dinucleotide Phosphate to Vertebrate Fatty Acid Synthetases
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منابع مشابه
Inhibition of rat liver nicotinamide adenine dinucleotide kinase by reduced nicotinamide adenine dinucleotide phosphate.
Rat liver NAD kinase (ATP : NAD 2’-phosphotransferase, EC 2.7.1.23) was purified about 70-fold. The MichaelisMenten constants (Km) for NAD and ATP were 8 x 10e4 M and 2 x low3 M, respectively. NAD kinase activity was markedly inhibited by NADH and also NADPH. The Ki of NADH was approximately 1 X 10q4 M, and that of NADPH was approximately 5 X 10M5 M. Both inhibitions were competitive with NAD, ...
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Apocynin (4-hydroxy-3-methoxyacetophenone), isolated from the traditional medicinal plant Picrorhiza kurroa, is a naturally occurring methoxy-substituted catechol, experimentally used as an inhibitor of reduced nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase.1 Activated neutrophil NADPH oxidase, a multisubunit complex composed of membrane-associated gp91phox (Nox2) and p22phox and c...
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As part of an investigation of the growth of tomato plants supplied with either nitrate or ammonium nitrogen (6), leaves were assayed for NAD4 and NADP. Anderson and Vennesland (1) assayed NAD and NADP in various green tissues and reported recoveries between 50 % and 85 % of NAD and NADP added to spinach leaf extracts. Very low levels of NAD and NADP were recorded for tomato leaf extracts. Thes...
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Incubation of NADH at neutral and slightly alkaline pH leads to the gradual absorption of 1 mol of H+. This uptake of acid requires oxygen and mainly yields anomerized NAD+ (NAD+), with only minimal formation od acid-modified NADH. The overall stoichiometry of the reaction is: NADH + H+ + 1/2O2 leads to H2O + NAD+, with NADH peroxide (HO2-NADH+) serving as the intermediate that anomerizes and b...
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Nicotiniamide adenine dintucleotide plhosphate-specific isocitrate dehydrogenase was extracted from etiolated pea (Pisurn satirum L.) seedlings and was purified 65-fold. The puirified enzyme exhibits one predoniinant protein band by pol acrylamide gel electrophoresis, w-hich corresponds to tiie dehy-drogenase activity as imieasuired by the nitro bluie tetrazolium technique. The reaction is read...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62462-7